Premium
UBER DIE FEHLENDE PHOTO— UND WIRTSZELLREAKTWIERBARKEIT DES UV‐INAKTIVIERTEN RNS‐PHAGEN FR
Author(s) -
Winkler Ulrich
Publication year - 1964
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1964.tb07848.x
Subject(s) - photolyase , rna , bacteria , extracellular , dna , intracellular , microbiology and biotechnology , biology , escherichia coli , enzyme , chemistry , dna repair , biochemistry , genetics , gene
. The plaque forming ability of the E. coli phage fr, containing single stranded RNA, is highly UV‐resistant. One lethal hit corresponds to approximately 1'2 × 10 5 erg cm ‐2 . photoreactivation of fr due to illumination of infected bacteria has not been found after UV‐inactivation of either extracellular or intracellular phage. This shows that the already known PR‐enzyme of E. coli K12 is probably specific only for some of the UV‐lesions in DNA but not in RNA. Furthermore, it may indicate that the photoreactivation of some RNA‐containing plant viruse (1,2) is due to a PR‐enzyme which is not present in K12. UV‐inactivated fr is also not host‐cell‐reactivable. The plaque forming ability of fr infected bacteria decreases rapidly with increasing doses of PR‐light wherea: the colony forming ability of uninfected host cells is very resistant to PR‐light.