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THE ACTION SPECTRUM OF AN IN VITRO DNA PHOTOREACTIVATION SYSTEM
Author(s) -
Setlow Jane K.,
Boling Maxon E.
Publication year - 1963
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1963.tb08904.x
Subject(s) - photolyase , action spectrum , flavin group , pyrimidine dimer , thymine , flavoprotein , dna , yeast , chemistry , in vitro , biophysics , photochemistry , biology , biochemistry , dna repair , enzyme
— The wavelength‐dependence of in vitro photoreactivation of transforming DNA by yeast extract has been determined. There is an intensity‐dependent lag at the beginning of the biological reaction. There is a similar lag in the splitting of thymine dimers by the yeast extract in the light, a process known to account for most or all of the increase in transforming activity of photoreactivated DNA. The most efficient wavelengths for photoreactivation are around 3550 and 3850 Å. Although the action spectrum is not very similar to flavin absorption, riboflavin at very low concentration inhibits photoreactivation, as it also inhibits a number of flavoenzymes, suggesting that the photoreactivating enzyme might be a flavoprotein.