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Isolation of bovine herpesvirus type 5 from the semen of a healthy bull in Australia
Author(s) -
Diallo IS,
Hewitson GR,
Hoad J,
Turner S,
Corney BG,
Rodwell BJ
Publication year - 2010
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.2009.00545.x
Subject(s) - amplicon , semen , virology , artificial insemination , biology , polymerase chain reaction , bovine herpesvirus 1 , insemination , isolation (microbiology) , virus , herpesviridae , microbiology and biotechnology , genetics , gene , viral disease , pregnancy
Artificial insemination is widely used in the cattle industry and a major challenge is to ensure that semen is free of infectious agents. A healthy donor bull was tested for freedom from infectious agents. A bovine herpesvirus was isolated in testis cells and identified as bovine herpesvirus type 5 (BoHV‐5) by polymerase chain reaction and by direct amplicon sequencing. The amplicon sequence shared 100% similarity with the published sequence of BoHV‐5. This is the first report in Australia of BoHV‐5 in semen. The implications of this finding are discussed.