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Detection of avian paramyxoviruses and influenza viruses amongst wild bird populations in Victoria
Author(s) -
PEROULIS I,
O'RILEY K
Publication year - 2004
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.2004.tb14650.x
Subject(s) - virology , biology , newcastle disease , influenza a virus subtype h5n1 , virus , hemagglutination assay , outbreak , hemagglutination , virulence , polymerase chain reaction , h5n1 genetic structure , equine influenza , infectious disease (medical specialty) , titer , gene , genetics , disease , covid-19 , medicine , pathology
Objective To isolate and characterise avian paramyx‐oviruses and other haemagglutinating viruses amongst Victorian wild bird populations. Procedure Tracheal and cloacal material was collected from wild duck, pigeon, quail and other wild birds throughout Victoria. Samples were processed and cultured in embryonating eggs. Viral isolates were characterised based on their haemagglutina‐tion and haemagglutination‐inhibition activity using a panel of specific antisera. Reverse transcriptase polymerase chain reaction and DNA sequencing were used to characterise Newcastle disease virus isolates. Results Twenty‐five nonpathogenic haemagglutinating viruses were isolated from 605 wild bird samples. The majority were characterised as APMV‐6 or influenza A virus, H3N2. Two isolates were identified and characterised as APMV‐1 (avirulent NDV) based on nucleotide and deduced amino acid sequence analysis at the F0 cleavage site. Conclusions Twenty‐five viruses were isolated, none of which resembled progenitor or virulent genotypes. This study provides valuable epidemiological information against which to compare future isolates from outbreaks of disease to determine their origin.