Premium
Comparison of three serological tests and an interferon‐g assay for the diagnosis of paratuberculosis in experimentally infected sheep
Author(s) -
GWOZDZ JM,
THOMPSON KG,
MURRAY A.,
REICHEL MP,
MANKTELOW BW,
WEST DM
Publication year - 2000
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.2000.tb10452.x
Subject(s) - paratuberculosis , serology , complement fixation test , immunodiffusion , biology , population , antibody , ouchterlony double immunodiffusion , virology , interferon gamma , mycobacterium , immunology , medicine , cytokine , bacteria , antiserum , genetics , environmental health
Objective To compare the diagnostic performance of a complement fixation test, an agar gel immunodiffusion test, an enzyme‐linked immunosorbent assay, and a whole‐blood interferon‐g assay for paratuberculosis in 14 sheep experimentally infected with Mycobacterium avium subsp paratuberculosis.Experimental design Longitudinal study. Results The IFN‐g assay detected more experimentally infected sheep, and earlier, than any of the serological tests. None of the antibody assays was able to detect all sheep with histologically confirmed paratuberculosis. Conclusions The superior performance of the IFN‐g assay in detecting infected sheep in this small experimental population warrants its further evaluation in a larger population of sheep naturally exposed to M a paratuberculosis .