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Distribution of G serotypes and P genotypes of bovine group A rotavirus isolated in Japan
Author(s) -
FUKAI K.,
SAKAI T.,
KAMATA H.
Publication year - 1998
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1998.tb12393.x
Subject(s) - rotavirus , serotype , virology , genotype , genotyping , biology , group a , polymerase chain reaction , reoviridae , microbiology and biotechnology , virus , medicine , gene , genetics
Objective To identify the distribution of G serotype and P genotype of bovine group A rotavirus in Japan. Design Detection, isolation, electropherotyping, G serotyping and P genotyping of bovine group A rotavirus in 167 diarrhoeal faecal samples. Procedure Bovine group A rotavirus was detected and isolated, and bovine group A rotavirus isolates were identified electropherotype by polyacrylamide gel electrophoresis and G serotype and P genotype by reverse transcription‐polymerase chain reaction. Results Twenty‐eight bovine group A rotavirus strains were isolated from 167 samples. Electropherotypes of the bovine group A rotavirus isolates were identified as long‐genome electropherotype and the most prevalent combination of G serotype and P genotype of the bovine group A rotavirus isolates was G6P5 (25/28; 89.3%), followed by G6P11 (2/28; 7.1%) and G10P11 (1/28; 3.6%). Conclusion These results suggest that the bovine group A rotavirus exhibiting G6P5 is the most common in Japan.