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Differentiation of Australian isolates of Mycobacterium paratuberculosis using pulsed‐field gel electrophoresis
Author(s) -
FEIZABADI MM,
ROBERTSON ID,
HOPE A,
COUSINS DV,
HAMPSON DJ
Publication year - 1997
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1997.tb11259.x
Subject(s) - paratuberculosis , typing , restriction enzyme , biology , strain (injury) , mycobacterium avium subsp. paratuberculosis , pulsed field gel electrophoresis , gel electrophoresis , veterinary medicine , dna profiling , northern territory , molecular epidemiology , microbiology and biotechnology , mycobacterium , virology , dna , genetics , genotype , bacteria , geography , gene , medicine , anatomy , archaeology
Objectives To examine strain variation amongst Australian isolates of Mycobacterium paratuberculosis . Design Pulsed field gel electrophoresis was optimised for differentiation of M paratuberculosis strains, and this typing technique was then applied to a collection of Australian isolates. Procedure DNAs from 35 Australian isolates of M para‐tuberculosis and a UK reference strain were digested with one or other of three restriction endonucleases. The banding patterns obtained after pulsed field gel electrophoresis of the DNA fragments were compared. Results The Australian isolates were divided into two groups on the basis of their DNA banding pattern. Both were different from the UK reference strain. Seven isolates from cattle in Victoria and the Northern Territory had the same pattern as five isolates from alpacas in Victoria and Western Australia. Another 20 isolates from cattle in Victoria, Western Australia and the Northern Territory had the same pattern as isolates from two sheep and a goat in New South Wales. Conclusion Pulsed field gel electrophoresis was a useful tool for strain typing of M paratuberculosis , and could be used to study the transmission of strains in Australia.