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Effect of adjuvants on antibody responses of sheep immunised with recombinant pili from Dichelobacter nodosus
Author(s) -
WALDUCK AK,
OPDEBEECK JP
Publication year - 1996
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1996.tb07568.x
Subject(s) - adjuvant , microbiology and biotechnology , pilus , antibody , dextran , emulsion , fimbria , biology , recombinant dna , immunology , escherichia coli , biochemistry , gene
Objective To compare the effects of two oil emulsion adjuvants (incomplete Freunds adjuvant and a proprietary oil adjuvant), DEAE‐dextran, L‐tyrosine particles and Quil A on the humoral immune responses of sheep immunised with recombinant pili of Dichelobacter nodosus (strain A). Procedure Antibody titres were studied for up to 32 weeks and were measured by bacterial agglutination and ELISA. The relative avidity of antibodies for pili was determined and the incidence and severity of adverse reactions at the site of injection of vaccines were recorded. Results The oil emulsion adjuvants and Quil A were more effective than either DEAE‐dextran or L‐tyrosine at stimulating antibodies in sheep. The incidence and severity of adverse reactions was lower in sheep which received vaccines containing either Quil A or DEAE‐dextran than in sheep which received vaccines containing oil emulsion adjuvants. L‐tyrosine had no adverse effects. Conclusion Quil A was as effective as oil adjuvants at stimulating high levels of antibodies against recombinant pili in sheep and had the significant advantage of being less irritant after subcutaneous injection.