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Development and trial of a bovine herpesvirus 1 ‐ thymidine kinase deletion virus as a vaccine
Author(s) -
SMITH GA,
YOUNG PL,
RODWELL BJ,
KELLY MA,
STORIE GJ,
FARRAH CA,
MATTICK JS
Publication year - 1994
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1994.tb03329.x
Subject(s) - virology , equine herpesvirus 1 , immunogenicity , thymidine kinase , biology , virus , restriction enzyme , bovine herpesvirus 1 , immune system , gene , recombinant dna , herpesviridae , herpes simplex virus , genetics , viral disease
SUMMARY An Australian bovine herpesvirus 1 (BHV1) isolate with a defined (427 base pair) deletion in the protein coding region of the thymidine kinase gene was obtained by standard marker rescue procedures. After selection in the presence of the nucleotide analogue 5iodo‐deoxy‐uridine the virus was analysed by hybridisation with three differential oligonucleotide probes, restriction endonuclease profile studies and DNA sequence analysis. The virus elicited an immune response in recipient animals after either intramuscular or intravenous administration and produced no significant deleterious side‐effects when administered at a dose sufficient to stimulate the host immune response. The safety and immunogenicity of the recombinant BHV1 virus 39B1 were similar to those reported for other registered BHV1 vaccines and the virus would appear to be suitable for the production of a vaccine seed lot and more exhaustive field trials as a prelude to commercial vaccine production and registration.