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Diagnosis by ELISA of bovine abortion due to Campylobacter fetus
Author(s) -
HUM S.,
STEPHENS LR,
QUINN C.
Publication year - 1991
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1991.tb03240.x
Subject(s) - campylobacteriosis , mucus , abortion , fetus , campylobacter fetus , antibody , biology , campylobacter , antigen , pregnancy , histopathology , microbiology and biotechnology , immunology , medicine , pathology , bacteria , ecology , genetics
SUMMARY An enzyme‐linked immunosorbent assay (ELISA) was developed to detect antigen‐specific secretory IgA antibody in bovine vaginal mucus after abortion due to Campylobacter fetus subsp venerealis. Abortions were diagnosed by isolating the organism from 8 foetuses and/or foetal membranes and by histopathology. Vaginal mucus was collected from 7 cows shortly after abortion. All showed a high level of IgA antibody in their vaginal mucus when they were compared with an uninfected control group. The new ELISA is simple and practical and provides a useful tool for diagnosis of bovine venereal campylobacteriosis.