Premium
Biological characterisation of Australian isolates of chicken anaemia agent
Author(s) -
CONNOR TJ,
McNEILLY F.,
FIRTH GA,
McNULTY MS
Publication year - 1991
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1991.tb03192.x
Subject(s) - polyclonal antibodies , biology , inoculation , immunofluorescence , monoclonal antibody , virology , antigen , antibody , indirect immunofluorescence , microbiology and biotechnology , immunology
SUMMARY Three Australian Isolates of chicken anaemia agent (CAA) resisted treatment at 70°C for 5 min and chloroform treatment. Although minor antigenic differences were detected using monoclonal antibodies to CAA, the Australian isolates were indistinguishable from the reference Cux‐1 and Gifu‐1 isolates in cross‐immunofluorescence and cross‐neutralisation tests employing polyclonal chicken antiserums. The Australian viruses were pathogenic for intramuscularly inoculated 1‐day‐old SPF chicks, but were less pathogenic for 7‐day‐old chicks. Thus the Australian isolates of CAA did not differ significantly in these properties from previously characterised CAA isolates from other continents.