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A sandwich enzyme immunoassay for bovine interferon‐γ and its use for the detection of tuberculosis in cattle
Author(s) -
ROTHEL JS,
JONES SL,
CORNER LA,
COX JC,
WOOD PR
Publication year - 1990
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1990.tb07730.x
Subject(s) - immunoassay , bovine tuberculosis , monoclonal antibody , interferon gamma , mycobacterium bovis , in vitro , recombinant dna , biology , virology , antigen , antibody , interferon , microbiology and biotechnology , chemistry , tuberculosis , immunology , medicine , biochemistry , mycobacterium tuberculosis , pathology , gene
SUMMARY An in vitro cellular assay for bovine tuberculosis has recently been developed. This assay detects gamma‐interferon released in response to specific antigen in a whole blood culture system. The bio‐assay previously described for the detection of bovine gamma‐interferon (IFN‐γ) has now been replaced with a sandwich enzyme immunoassay (EIA) which utilises two monoclonal antibodies to bovine IFN‐γ. The EIA detects less than 25pg/ml of recombinant bovine IFN‐γ and is specific for biologically active bovine IFN‐γ; and does not detect bovine alpha or beta interferon. IFN‐γ from sheep, goat and buffalo, but not from pig, deer or man, are also recognised by the EIA. The bovine IFN‐γ EIA when used in conjunction with the whole blood culture system has resulted in a simple, rapid and sensitive in vitro assay for specific cell mediated immune responsiveness to M. bovis infection in cattle.