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Protection against enzootic pneumonia of pigs: intraperitoneal inoculation with live LKR strain of Mycoplasma hyopneumoniae
Author(s) -
LLOYD LC.,
COTTEW GS.,
ANDERSON DA.
Publication year - 1989
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1989.tb09704.x
Subject(s) - mycoplasma hyopneumoniae , enzootic , pneumonia , microbiology and biotechnology , strain (injury) , inoculation , virology , biology , medicine , veterinary medicine , immunology , anatomy , virus
Pigs obtained from a mycoplasma‐free piggery were randomised into 4 groups of 9. Groups 1 and 2 were injected by the intraperitoneal route with liquid culture of the LKR strain of Mycoplasma hyopneumoniae. Group 1 was injected once and group 2 twice. Group 3 was made up of pigs inoculated by the intranasal route with the virulent Beaufort strain of M. hyopneumoniae ; they served as the source of infection for the challenge. Group 4 were uninfected, uninjected controls. Six weeks after the last injection, groups from 1 to 4 were placed in contact. Seven of the pigs in the 1‐dose group and 6 in the 2‐dose group were free of lesions at necropsy 6 weeks after challenge. Of the two pigs with lesions in the 1‐dose group one had only a small lesion but the other had extensive lesions; it had not shown an antibody response after injection of culture. The lesions in the 3 pigs in the 2‐dose group were all small. All 9 control pigs had lesions which varied from medium to large in size. The difference in the incidence of pneumonia between the injected and control groups was significant (P < 0.05) and the proportion of severely affected pigs in the vaccinated groups was significantly lower (P > 0.01). There was no difference between those given one dose of vaccine and those receiving 2 doses.