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Evaluation of an enzyme‐linked immunosorbent assay for the detection of Corynebacterium pseudotuberculosis infection in sheep
Author(s) -
SUTHERLAND S S,
ELLIS T M,
MERCY A R,
PATON M,
MIDDLETON H
Publication year - 1987
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1987.tb15953.x
Subject(s) - corynebacterium pseudotuberculosis , antigen , biology , antibody , microbiology and biotechnology , enzyme , virology , immunology , bacteria , biochemistry , genetics
SUMMARY: :Two enzyme‐linked immunosorbent assays to measure antibody to the cell wall antigen (C‐ELISA) and toxin antigen (T‐ELISA) of C. pseudotuberculosis were evaluated on serum from 6 separate groups of sheep. For sheep naturally infected with C. pseudotuberculosis the sensitivity and specificity of the C‐ELISA was 76% and 73% respectively and for the T‐ELISA 67% and 77% respectively. For sheep slaughtered one year after artificial infection the sensitivity of both tests was 83% and the specificity 72%. For sheep slaughtered 4 months after artificial infection the specificity of both tests was < 30% while the mean sensitivity was 85%. The C‐ELISA in conjunction with the T‐ELISA detected 92% of sheep with lung lesions. Aust Vet J 64: 263–266