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A technique for the purification of Mycobacterium paratuberculosis from the ileal mucosa of infected cattle
Author(s) -
RATNAMOHAN T N,
SPENCER T L
Publication year - 1986
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1986.tb02970.x
Subject(s) - paratuberculosis , microbiology and biotechnology , antigen , biology , deoxyribonuclease , lysozyme , differential centrifugation , mycobacterium , bacteria , intestinal mucosa , centrifugation , immunology , enzyme , medicine , biochemistry , genetics
SUMMARY Mycobacterium paratuberculosis , the causative agent of paratuberculosis, produces considerable economic loss in the cattle industry in many countries. The slow growth of M. paratuberculosis has hindered investigations of the antigenic composition of the organism and the development of species‐specific antigen for serological detection of this disease. This paper describes a simple method for the isolation of large quantities of viable M. paratuberculosis from the intestinal mucosa of infected cattle by a combination of trypsin digestion, deoxyribonuclease/lysozyme treatment and differential centrifugation. Purity was about 99% and yield between 10 5 –10 9 bacteria/g tissue.