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A microplate enzyme‐linked immunorsorbent assay for measuring antibody to Anaplasma marginale in cattle serum
Author(s) -
BARRY D N,
PARKER R J,
VOS A J,
DUNSTER P.,
RODWELL B J
Publication year - 1986
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1986.tb02934.x
Subject(s) - antibody , complement fixation test , babesia bovis , titer , virology , biology , anaplasmosis , antigen , babesiosis , tick , immunology , serology
SUMMARY An enzyme‐linked immunorsorbent assay (ELISA) method is described for measuring antibody against Anaplasma marginale in cattle serum. This method was more sensitive and objective than a previously described ELISA method for A. marginale and possible reasons for this are discussed. All 83 cattle experimentally infected with A. marginale (81) or A. centrale (2) developed demonstrable specific antibody but the serums of 98.8% of 839 cattle from cattle tick‐free areas did not react by ELISA; 378 serums containing antibody to Babesia bovis were tested for cross reactions in the A. marginale ELISA. There were no significant cross‐reactions except when cattle had been inoculated at least twice with B. bovis ‐infected erythrocytes, presumably due to antibodies reacting with erythrocyte material in the ELISA antigen. The ELISA detected antibodies for more than 3 years after infection, at least 2 years longer than did a complement fixation test. When A. marginale infections in cattle were eliminated by long acting oxytetracycline, their serums ceased to react by ELISA. An ELISA score for serum antibody level was shown to have a statistically significant correlation with ELISA titre.