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An enzyme‐linked immunosorbent assay for detection of Brucella abortus in cattle using monoclonal antibodies
Author(s) -
SUTHERLAND S.
Publication year - 1985
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1985.tb14248.x
Subject(s) - monoclonal antibody , horseradish peroxidase , vaccination , brucella abortus , antibody , biology , strain (injury) , group b , brucellosis , cattle diseases , virology , brucella , enzyme , veterinary medicine , microbiology and biotechnology , medicine , immunology , biochemistry , anatomy
SUMMARY: One group of 24 cattle was vaccinated with the usual calfhood dose of B. abortu s strain 19 and a further 27 cattle were similarly vaccinated but as adults. Twenty four cattle (12 from each group) and a control group of 12 cattle were subsequently challenged with B. abortus strain 544. Two monoclonal antibodies (MA (A) and MA (B)) conjugated to horseradish peroxidase were used independently in a competitive enzyme‐linked immunosorbent assay (ELISA) to test the serums. After vaccination with B. abortus strain 19, the performance of the monoclonal antibodies was in general agreement with the CFT as fewer calfhood vaccinates were positive 12 weeks after vaccination to the ELISA with MA (A) and MA (B) than adult vaccinates. After challenge, MA (A) and MA (B) ELSA tests detected the infected cattle earlier than the CFT, but more positive reactions occurred in the cattle that proved uninfected at slaughter. Aust. vet. J. 62: 264–268