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A urease‐ELISA for the detection of mycoplasma infections in poultry
Author(s) -
PATTEN B. E.,
HIGGINS P. A.,
WHITHEAR K. G.
Publication year - 1984
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1984.tb07219.x
Subject(s) - mycoplasma gallisepticum , urease , conjugate , antigen , agglutination (biology) , haemagglutination inhibition , direct agglutination test , hemagglutination , microbiology and biotechnology , chemistry , antibody , biology , chromatography , mycoplasma , virology , enzyme , immunology , biochemistry , serology , mathematical analysis , mathematics
SUMMARY An ELISA utilising a urease‐antibody conjugate specific to chicken IgG was examined as an alternative to the serum agglutination and the haemagglutination inhibition tests in the diagnosis of Mycoplasma gallisepticum and M. synoviae infections in poultry. Use of a urease conjugate allowed the serum reactions to be appraised without the need for expensive photometric equipment. Non‐specific binding of conjugate to antigen was eliminated by treatment of antigen coated microplates with 10% foetal calf serum in phosphate buffered saline. Some chicken serums produced non‐specific reactions. These reactions were reduced without any loss of test sensitivity by making the initial 1:5 dilution of chicken serum in whole sheep serum rather than diluting buffer. Tests on serums from experimentally infected chickens showed that the urease ELISA was specific, and was as sensitive as the serum agglutination test but more sensitive than the haemagglutination inhibition test.