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THE ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA) AS A SEROLOGICAL TEST FOR DETECTING ANTIBODIES AGAINST LEPTOSPIRA INTERROGANS SEROVAR HARDJO IN SHEEP
Author(s) -
Adler B.,
Faine S.,
Gordon L. M.
Publication year - 1981
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1981.tb00546.x
Subject(s) - serotype , serology , leptospira interrogans , group b , antibody , virology , biology , leptospira , microbiology and biotechnology , medicine , immunology
SUMMARY: The enzyme‐linked immunosorbent assay (ELISA) was compared with the standard microscopic agglutination test (MAT) as a method for detecting antibodies against Leptospira interrogans serovar hardjo in sheep. Peak antibody levels detected by the 2 tests occurred at different times following experimental infection of sheep. In serums from flocks of sheep with naturally acquired infection there was a 95% correlation between MAT and ELISA with respect to the presence or absence of antibody to serovar hardjo , although the level of correlation of the titres of the 2 tests was low. The 2 tests appeared to measure different antigen‐antibody systems. The ELISA would be a useful test for screening large numbers of serums for antibodies to L. interrogans serovar hardjo .