Premium
AN IMMUNOFLUORESCENCE DIAGNOSTIC TEST FOR AVIAN INFECTIOUS LARYNGOTRACHEITIS
Author(s) -
Wilks C. R.,
Kogan V. G.
Publication year - 1979
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1979.tb15864.x
Subject(s) - direct fluorescent antibody , virology , immunofluorescence , antiserum , antibody , antigen , biology , tracheitis , virus , microbiology and biotechnology , immunology , ecology , bronchitis
SUMMARY: An antiserum to a recent field isolate of infectious laryngotracheitis virus was conjugated with fluorescein isothiocyanate and used to detect viral antigen to infected chorioallantoic membranes and trachael epithelium by the direct fluorescent antibody technique. In experimentally infected birds, viral antigen was detected with the fluorescent antibody technique from 2 to 14 days post‐inoculation but histological evidence of tracheitis was only observed from day three to day ten. The fluorescent antibody test detected 22 of 23 histolgically confirmed cases of ILT and was more accurate than virus isolation when used in the diagnosis of respiratory disease from field outbreaks. It was concluded that the speed, accuracy and sensitivity of the fluorescent antibody technique make it a useful tool in the diagnosis of infectious laryngotracheitis.