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EVALUATION OF NEW AND CURRENTLY USED DIAGNOSTIC PROCEDURES FOR BOVINE BRUCELLOSIS
Author(s) -
Sutherland S. S.,
Cras D. V. Le
Publication year - 1978
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1978.tb02481.x
Subject(s) - complement fixation test , brucellosis , direct agglutination test , medicine , haemolysis , titer , latex fixation test , brucella melitensis , brucella , vaccination , immunology , antibody , veterinary medicine , biology , serology
SUMMARY The indirect haemolysis test (IHLT) and the rough antigen complement‐fixation test (RCFT) were compared with several conventional tests using serum samples from 9 cows known to be infected with Brucella abortus. In 7 cows all the tests except the RCFT (which was developed to detect antibodies resulting from 45/20 vaccination) became positive and remained so until the cows were autopsied 6 months after infection. In the other 2 cows the Rose Bengal test was occasionally negative and the titre of the serum agglutination test only reached 100 iu briefly. The routine complement‐fixation test, the IHLT and the anti‐bovine globulin test reached diagnostic levels at most of the weekly test intervals but occasional low titres occurred in them all. Only very low or negative titres were detected by the RCFT.