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THE SEROLOGICAL DIAGNOSIS OF BOVINE BRUCELLOSIS: AN EVALUATION OF THE COMPLEMENT FIXATION, SERUM AGGLUTINATION AND ROSE BENGAL TESTS
Author(s) -
Alton G. G.,
Maw J.,
Rogerson B. A.,
McPherson G. G.
Publication year - 1975
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/j.1751-0813.1975.tb09404.x
Subject(s) - rose bengal , brucellosis , complement fixation test , serology , bovine brucellosis , agglutination (biology) , medicine , virology , immunology , veterinary medicine , biology , brucella abortus , antibody , genetics
In a collaborative investigation in which 4 laboratories took part, the Rose Bengal Test (RBT), Serum Agglutination Test (SAT) and 4 different Complement Fixation (CFT) techniques were evaluated in selected cattle for the diagnosis of bovine brucellosis, by comparing the results they gave with the bacteriological examination of a selection of lymph nodes taken from the same animals at slaughter. The RBT correctly classified all but 1 of 79 culture-positive cattle, but was more often positive in culture-negative animals than the other tests. The RBT may be most useful as a screen test. 11% of the culture-positive cattle had SAT titres below 100 iu and almost 4% of them had less than 30 iu. However, the SAT was more effective in cattle vaccinated with the 45/20 vaccine. The CFT, in 1 of 4 techniques used, identified all of the culture-positive cattle at a serum dilution of 1/4 or above and was considered to be far superior to the SAT as a diagnostic test, except perhaps in cattle vaccinated with the 45/20 vaccine.