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Evaluation Antibacterial Activity of Quaternary‐Based Chitin/Chitosan Derivatives In Vitro
Author(s) -
Geng Xiaodong,
Yang Ronghua,
Huang Jianying,
Zhang Xiao,
Wang Xiangyang
Publication year - 2013
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2012.02999.x
Subject(s) - chitin , chitosan , bacillus subtilis , antibacterial activity , antimicrobial , polysaccharide , chemistry , minimum inhibitory concentration , bacteria , nuclear chemistry , food science , microbiology and biotechnology , biochemistry , organic chemistry , biology , genetics
Total of 3 water‐soluble quaternary‐based chitin/chitosan derivatives, which have an identical molecular weight and anion, were synthesized and characterized. Their antibacterial activities against Salmonella cholerae‐suis and Bacillus subtilis were evaluated in vitro . The polysaccharides exhibited the antibacterial efficiency. Their minimum inhibitory concentration (MIC) values vary from 0.02 to 20.48 mg/mL, and their minimum bactericidal concentration (MBC) values vary from 0.08 to 40.96 mg/mL against S. cholerae‐suis and B. subtilis , respectively. Futhermore, the extent of Bacillus subtilis cells damage was examined via transmission electron microscopy (TEM) to show how N,N,N ‐trimethylchitosan (TMC) gradually destroyed and killed B. subtilis cells when they were treated with TMC. One of those quaternary polymers, O ‐([2‐hydroxy‐3‐trimethylammonium])propyl chitin (OHT‐chitin), which can be directly and easily synthesized from chitin in bulk quantities, also was demonstrated its antibacterial activity. These water soluble quaternary‐based chitin/chitosan derivatives that have antibacterial effect should be potentially used as antimicrobial agents in many fields. Practical Application: The main practical application behind the investigation and evaluation antibacterial activity of 3 water‐soluble quaternary‐based chitin/chitosan derivatives could be potentially used as antimicrobial agents in many fields. These polysaccharides represent a renewable source of natural biodegradable polymers and meet with the emergence of more and more food safe problems.