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Comparison of Decontamination Efficacy of Antimicrobial Treatments for Beef Trimmings against Escherichia coli O157:H7 and 6 Non‐O157 Shiga Toxin‐Producing E. coli Serogroups
Author(s) -
Geornaras Ifigenia,
Yang Hua,
Manios Stavros,
Andritsos Nikolaos,
Belk Keith E.,
Nightingale Kendra K.,
Woerner Dale R.,
Smith Gary C.,
Sofos John N.
Publication year - 2012
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2012.02878.x
Subject(s) - sodium chlorite , human decontamination , antimicrobial , escherichia coli , chemistry , food science , sodium , microbiology and biotechnology , pathogen , inoculation , shiga toxin , biology , chlorine dioxide , biochemistry , medicine , horticulture , organic chemistry , pathology , gene , inorganic chemistry
The decontamination efficacy of 6 chemical treatments for beef trimmings were evaluated against Escherichia coli O157:H7 and 6 non‐O157 Shiga toxin‐producing E. coli (nSTEC) serogroups. Rifampicin‐resistant 4‐strain mixtures of E. coli O157:H7 and nSTEC serogroups O26, O45, O103, O111, O121, and O145 were separately inoculated (3 to 4 log CFU/cm 2 ) onto trimmings (10 × 5 × 1 cm; approximately 100 g) fabricated from beef chuck rolls, and were immersed for 30 s in solutions of acidified sodium chlorite (0.1%, pH 2.5), peroxyacetic acid (0.02%, pH 3.8), sodium metasilicate (4%, pH 12.5), Bromitize ® Plus (0.0225% active bromine, pH 6.6), or AFTEC 3000 (pH 1.2), or for 5 s in SYNTRx 3300 (pH 1.0). Each antimicrobial was tested independently together with an untreated control. Results showed that all tested decontamination treatments were similarly effective against the 6 nSTEC serogroups as they were against E. coli O157:H7. Irrespective of pathogen inoculum, treatment of beef trimmings with acidified sodium chlorite, peroxyacetic acid, or sodium metasilicate effectively ( P < 0.05) reduced initial pathogen counts (3.4 to 3.9 log CFU/cm 2 ) by 0.7 to 1.0, 0.6 to 1.0, and 1.3 to 1.5 log CFU/cm 2 , respectively. Reductions of pathogen counts (3.1 to 3.2 log CFU/cm 2 ) by Bromitize Plus, AFTEC 3000, and SYNTRx 3300 were 0.1 to 0.4 log CFU/cm 2 , depending on treatment. Findings of this study should be useful to regulatory authorities and the meat industry as they consider nSTEC contamination in beef trimmings. Practical Applications: Findings of this study should be useful to: (i) meat processors as they design and conduct studies to validate the efficacy of antimicrobial treatments to control pathogen contamination on fresh beef products; and (ii) regulatory agencies as they consider approaches for better control of the studied pathogens.