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Separation of Phospholipids from Hen Egg Yolk by Short Packed Silica Gel Column Chromatography
Author(s) -
Lei Lin,
Li Jing,
Hu Jiangning,
Liu Rong,
Fan Yawei,
Tang Liang,
Deng Zeyuan
Publication year - 2012
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2012.02850.x
Subject(s) - chromatography , chemistry , silica gel , high performance liquid chromatography , yolk , phosphatidylethanolamine , phosphatidylcholine , ethanol , elution , packed bed , phospholipid , biochemistry , food science , membrane
Short packed silica gel column chromatography has been performed to optimize the production of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) from hen egg yolk with very low or no toxic solvents. The effects of silica type, sample loading amount, dimension of the glass chromatotube, and mobile phase compositions were investigated and high separation efficiency was achieved: gradient elution as 200 mL ethanol followed by 300 mL 95% ethanol to fractionate PE and PC after neutral lipids (NL) removed by 120 mL ethyl acetate, 40 mm silica gel (54 to 74 μm) bed height of the chromatotube with 22 mm inner dia (ID), and 0.25 g sample loading amount. By this procedure, 3.69 g PE and 2.88 g PC per 100 g egg yolk lipids were obtained, respectively. The refined PE and PC were identified by high‐performance liquid chromatography/ultraviolet detector (HPLC‐UV) with purity over 96%. The fatty acids in egg yolk revealed that PE and PC characterized higher ratios of n − 6/ n − 3 (PE, 7.41; PC, 8.99). 18:2 n − 6 of PC (15.21%) predominated over PE (10.29%), whereas the level of 20:4 n − 6 of PC (8.78%) was lower than PE (15.67%).