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Shrimp Waste Extract and Astaxanthin: Rat Alveolar Macrophage, Oxidative Stress and Inflammation
Author(s) -
Santos Suzan D.,
Cahú Thiago B.,
Firmino Guilherme O.,
de Castro Célia C.M.M.B.,
Carvalho Jr. Luiz B.,
Bezerra Ranilson S.,
Filho José L. Lima
Publication year - 2012
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2012.02762.x
Subject(s) - astaxanthin , shrimp , antioxidant , butylated hydroxytoluene , chemistry , superoxide dismutase , oxidative stress , biochemistry , nitric oxide , food science , superoxide , pharmacology , carotenoid , biology , enzyme , fishery , organic chemistry
Astaxanthin is a carotenoid known to have antioxidant and antiinflammatory properties. This study examined if shrimp astaxanthin modulates the production of superoxide (O − 2 ), nitric oxide (NO), and tumor necrosis factor‐α (TNF‐α) in rat alveolar macrophages. The oxidative effect was induced by phorbol myristate acetate and lipopolysacharide. The treatment was compared with superoxide dismutase, butylated hydroxytoluene, commercial astaxanthin, N‐nitric‐L‐arginine methyl ester and L‐ canavanine, all administered as a 43.5‐μg/mL dose in the presence of 1% EtOH/0.5% DMSO. All treatments maintained cell viability, as observed in the MTT assay, and shrimp extract increased the viable alveolar macrophages to 168%. Shrimp extract and commercial astaxanthin showed a suppressive effect on the generation of both free radicals O − 2 and NO, while purified shrimp astaxanthin was specific to NO. TNF‐α secretion was correlated with NO production. However, in this correlation, the shrimp extract completely inhibited TNF‐α. In the light of these findings, the antioxidant action demonstrated in this study suggests that the shrimp extract could be considered as a promising source of bioactive substances with antioxidant and anti‐inflammatory activity. Practical Application: The hydrolysis process of shrimp waste generates bioactive products that add economic value to shrimp processing, mainly because they may have applications in nutraceutical and animal feed industry.