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Identification and Characterization of Gamma‐Irradiated Dried Lentinus edodes Using ESR, SEM, and FTIR Analyses
Author(s) -
Akram Kashif,
Ahn JaeJun,
Kwon JoongHo
Publication year - 2012
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2012.02740.x
Subject(s) - fourier transform infrared spectroscopy , irradiation , lentinus , electron paramagnetic resonance , mushroom , nuclear chemistry , sugar , materials science , chemistry , scanning electron microscope , food science , nuclear magnetic resonance , chemical engineering , composite material , physics , nuclear physics , engineering
A detailed electron spin resonance (ESR) analysis for different parts of gamma‐irradiated (0 to 50 kGy) dried mushroom ( Lentinus edodes ) was conducted to identify radiation‐induced signals. All studied mushroom parts except gills produced strong dose‐dependent radiation‐induced ESR signals particularly at about g = 2.0076, 20005, and 1.9911 demonstrating the generation of crystalline sugar radicals following irradiation. The intensity of these signals was highest in cap skin samples, followed by the cap core, stem skin, whole mushroom powder, and stem core samples, respectively. ESR‐based identification was easily possible at 2 kGy or more using mushroom cap skin or cap core as samples. The radiation‐induced ESR signals were found sensitive to thermal treatment showing the limitation of ESR‐based detection in case of heat‐processed samples. Scanning electron microscopy (SEM) showed micro‐structural damage upon irradiation resulting decreased percentage of rehydration; however, no major alteration was observed through the Fourier transform infrared (FTIR) spectra. Irradiation changed the structural morphology; however, the main functional groups were stable against radiation treatment. Practical Application: Food irradiation can confirm hygienic quality and prolong the product shelf life. However, due to lack of international consensus, effective identification methods and detailed quality characterization are required for the general use of this technology.