Premium
Development and Application of an Enzyme‐linked Immunosorbent Assay for Specific Detection of Mangiferin Content in Various Cultivars of Mangifera indica Leaves Using Anti‐mangiferin Polyclonal Antibody
Author(s) -
Yusakul Gorawit,
Kitirattrakarn Wongsathorn,
Tanwanichkul Narunat,
Tanaka Hiroyuki,
Putalun Waraporn
Publication year - 2012
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2011.02610.x
Subject(s) - mangiferin , mangifera , cultivar , chemistry , nutraceutical , polyclonal antibodies , hesperidin , xanthone , food science , chromatography , horticulture , traditional medicine , biology , antibody , medicine , alternative medicine , pathology , stereochemistry , immunology
An enzyme‐linked immunosorbent assay (ELISA) was developed for determining mangiferin content in plant samples using a polyclonal antibody (PAb) against mangiferin. The developed ELISA showed a full measurement range from 0.12 to 31.25 μg/mL mangiferin with a relative standard deviation (RSD) less than 6% for both intra‐ and interassay precision levels. The accuracy was determined by a percent recovery experiment at three concentration levels and it showed 97.8%–103.7% recovery in Mangifera indica leaf samples. The developed ELISA exhibited a high correlation value ( R 2 = 0.992) with the standard high‐performance liquid chromatography (HPLC) method in various mangiferin‐containing plant samples. Our results suggest that the validated ELISA methodology using a PAb against mangiferin can be applied to determine mangiferin content with high specificity, rapidity and simplicity in various mangiferin‐containing plant samples. The mangiferin content in the mature leaves of fifty M. indica cultivars were determined using the developed ELISA. The mangiferin contents ranged from 1.94 ± 0.13% to 13.79 ± 0.84% dry wt. The Thawai cultivar leaves contained the highest level of mangiferin (13.79 ± 0.84% dry wt), but it is a rare cultivar. The Namdokmai, which is more commonly cultivated in Thailand, contain 12.41 ± 0.60% dry wt mangiferin; therefore, this cultivar leaf was recommended as the source of raw material for the pharmaceutical, nutraceutical and cosmetic product industries. Practical Application: Currently, natural heath products are accepted worldwide for healthcare. Mangiferin‐containing plants and products exhibit health benefits against oxidative stress‐related diseases, such as cardiovascular diseases, cancer, dyslipidemia and diabetes. We have developed an ELISA with high specificity, rapidity and simplicity for the quality control of mangiferin‐derived product production. Moreover, we found that the Namdokmai leaf, a Thai M. indica cultivar, was recommended as the source of raw material for the pharmaceutical, nutraceutical and cosmetic product industry because of its high mangiferin content and natural prevalence.