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Lipase‐Catalyzed Production of Pinolenic Acid Concentrate from Pine Nut Oil Using a Recirculating Packed Bed Reactor
Author(s) -
Zhao TingTing,
Kim Byung Hee,
Hong Seung In,
Yoon Sung Won,
Kim ChongTai,
Kim Yangha,
Kim InHwan
Publication year - 2012
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2011.02562.x
Subject(s) - chemistry , lipase , yield (engineering) , candida antarctica , nut , molar ratio , ethanol , catalysis , residence time (fluid dynamics) , substrate (aquarium) , fatty acid , chromatography , nuclear chemistry , organic chemistry , food science , enzyme , materials science , biology , ecology , geotechnical engineering , structural engineering , engineering , metallurgy
Pinolenic acid (PLA) concentrate in fatty acid ethyl ester (FAEE) was efficiently produced from pine nut oil via lipase‐catalyzed ethanolysis using a recirculating packed bed reactor (RPBR). The effects of reaction temperature, molar ratio, and residence time on the concentration of PLA were explored. Novozym 435 lipase from Candida antarctica showed less selectivity toward PLA esterified at the sn ‐3 position when temperature was increased from 45 to 55 °C. For the trials of molar ratio between 1: 50 and 1: 100 (pine nut oil to ethanol), there were no significant differences in the yield of PLA. Residence time of substrate in a RPBR affected significantly the PLA content as well as the yield of PLA. Optimal temperature, molar ratio (pine nut oil to ethanol), and residence time for production of PLA concentrate via lipase‐catalyzed ethanolysis in a RPBR were 45 °C, 1: 50, and 3 min, respectively. Under these conditions, the maximal PLA content (36.1 mol%) in the concentrate was obtained during the initial 10 min of reaction.