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A Kinetic Study on Sesame Cake Protein Hydrolysis by Alcalase
Author(s) -
Demirhan Elçin,
Apar Dilek Kılıç,
Özbek Belma
Publication year - 2010
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2010.01938.x
Subject(s) - bacillus licheniformis , chemistry , hydrolysate , hydrolysis , protease , enzymatic hydrolysis , hydrolyzed protein , kinetics , chromatography , substrate (aquarium) , rice protein , enzyme , food science , biochemistry , bacteria , genetics , physics , oceanography , quantum mechanics , bacillus subtilis , biology , geology
In the present study, the hydrolysis of sesame cake protein was performed by Alcalase, a bacterial protease produced by Bacillus licheniformis , to investigate the reaction kinetics of sesame cake hydrolysis and to determine decay and product inhibition effects for Alcalase. The reactions were carried out for 10 min in 0.1 L of aqueous solutions containing 10, 15, 20, 25, and 30 g protein/L at various temperature and pH values. To determine decay and product inhibition effects for Alcalase, a series of inhibition experiments were conducted with the addition of various amounts of hydrolysate. The reaction kinetics was investigated by initial rate approach. The initial reaction rates were determined from the slopes of the linear models that fitted to the experimental data. The kinetic parameters, K m and V max , were estimated as 41.17 g/L and 9.24 meqv/L.min. The Lineweaver–Burk plots showed that the type of inhibition for Alcalase determined as uncompetitive, and the inhibition constant, K i , was estimated as 38.24% (hydrolysate/substrate mixture). Practical Application: Plant proteins are increasingly being used as an alternative to proteins from animal sources to perform functional roles in food formulation. Knowledge of the kinetics of the hydrolysis reaction is essential for the optimization of enzymatic protein hydrolysis and for increasing the utilization of plant proteins in food products. Therefore, in the present study, the hydrolysis of sesame cake protein was performed by Alcalase, a bacterial protease produced by B. licheniformis , to investigate the reaction kinetics of sesame cake hydrolysis and to determine decay and product inhibition effects for Alcalase.