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Natural Flavone Kaempferol Suppresses Chemokines Expression in Human Monocyte THP‐1 Cells through MAPK Pathways
Author(s) -
Huang ChingHua,
Jan RenLong,
Kuo ChangHung,
Chu YuTe,
Wang WeiLi,
Lee MinSheng,
Chen HuanNan,
Hung ChihHsing
Publication year - 2010
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2010.01812.x
Subject(s) - chemokine , kaempferol , mapk/erk pathway , chemistry , monocyte , lipopolysaccharide , nf κb , ccl7 , signal transduction , immunology , cancer research , biology , cxcl10 , immune system , biochemistry , flavonoid , antioxidant
There is increasing evidence that daily intake of flavonoids reduced severity and prevalence of allergic diseases. However, the mechanism of its antiinflammatory effects in allergic diseases remains uncertain. Kaempferol, which belongs to the flavone group, is a strong antioxidant among natural flavonoids and is the essential component of many beverages and vegetables. Because chemokine is one of the key mediators in allergic inflammatory process, we investigated the effect of kaempferol on chemokines expression in monocytes. Our data demonstrated that kaempferol significantly inhibited the lipopolysaccharide (LPS)‐induced production of monocyte‐derived chemokine (MDC), interferon gamma‐induced protein 10 (IP‐10), and interleukin‐8 (IL‐8) in THP‐1 cells. Growth‐related oncogene‐α (GRO‐α) was also suppressed at a higher concentration. We also found that kaempferol was able to suppress LPS‐induced mitogen‐activated protein kinase (MAPK) pathways, as well as the phosphorylation of upstream c‐raf and MEK1/2. In brief, kaempferol suppressed LPS‐induced T helper 1 (Th1), T helper 2 (Th2), and neutrophil‐related chemokines production in monocytes might be via the MAPK pathways.