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Optimization of Hydrolysis Conditions for the Production of Antioxidant Peptides from Fish Gelatin Using Response Surface Methodology
Author(s) -
You Lijun,
Regenstein Joe M.,
Liu Rui Hai
Publication year - 2010
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2010.01707.x
Subject(s) - gelatin , response surface methodology , hydrolysis , antioxidant , fish <actinopterygii> , chemistry , food science , production (economics) , biochemistry , chromatography , fishery , biology , macroeconomics , economics
Fish skin gelatin was hydrolyzed with papain to produce antioxidant peptides. Response surface methodology (RSM) was applied to optimize the hydrolysis conditions (including enzyme to substrate ratio [E/S], hydrolysis time, and temperature). The highest degree of hydrolysis (DH) (50.1 ± 1.1%) was obtained at an E/S of 2% at 56.8 °C, 2.11 h, and was not significantly different from the predicted values within a 95% confidence interval. The highest 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) (96.8 ± 0.9%) and 2,2′‐azinobis (3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt (ABTS •+ ) (9.80 ± 0.11 mM Trolox [6‐hydroxy‐2,5,7,8‐tetramethychroman‐2‐carboxylic acid]) radical‐scavenging activities of fish gelatin hydrolyzates were obtained at an E/S of 3% at 52.1 °C, 2.65 h, and both DPPH and ABTS •+ radical‐scavenging activities were not significantly different from the predicted values 97.3% and 9.86 mM Trolox within the 95% confidence interval. Therefore, RSM is an efficient way to optimize fish gelatin hydrolysation and the resultant hydrolyzates show promise as antioxidant peptides. Practical Application: There is a growing interest in the use of fish gelatin as an alternative to mammalian gelatin. One potential use is as a source of widely acceptable functional compounds. In this study, a search for antioxidant peptides from fish gelatin prepared by an enzymatic method has been successfully done. This suggests that this is a practical way to obtain bioactive peptides.