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Polyphenolic Acids from Mint (the Aerial of Mentha haplocalyx Briq.) with DPPH Radical Scavenging Activity
Author(s) -
She G.M.,
Xu C.,
Liu B.,
Shi R.B.
Publication year - 2010
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2010.01603.x
Subject(s) - rosmarinic acid , chemistry , dpph , polyphenol , antioxidant , scavenging , acetone , chromatography , stereochemistry , organic chemistry
Vegetables of mint (the aerial part of Mentha haplocalyx ) contain a significant amount of polyphenols with many health benefits. The crude aqueous acetone extract exhibited high antioxidant activity (IC 50 = 45.67 μg/mL) in 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging assay. The activity‐guided separation of chlorophyll removal fraction on column chromatography afforded 8 polyphenolic acids, including a new compound, cis ‐salvianolic acid J ( 1 ), and 7 known compounds, salvianolic acid J ( 2 ), lithospermic acid ( 3 ), rosmarinic acid ( 4 ), lithospermic acid B ( 5 ), magnesium lithospermate B ( 6 ), sodium lithospermate B ( 7 ), and danshensu ( 8 ), respectively. Their structural elucidations of all the compounds were based on extensive spectroscopic methods, including HRESIMS and 2D NMR experiments (HSQC, HMBC, and ROESY) and by comparison with reference values. Compounds 2 , 3 , and 5 to 8 were isolated from Mentha genus for the 1st time. The DPPH radical scavenging activities of all the isolated compounds were evaluated.