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Development and Validation of a Matrix Solid‐Phase Dispersion Method to Determine Acrylamide in Coffee and Coffee Substitutes
Author(s) -
Soares Cristina M. Dias,
Alves Rita C.,
Casal Susana,
Oliveira M. Beatriz P.P.,
Fernandes José Oliveira
Publication year - 2010
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2010.01545.x
Subject(s) - chromatography , acrylamide , sorbent , matrix (chemical analysis) , extraction (chemistry) , elution , chemistry , solid phase extraction , dispersion (optics) , sample preparation , organic chemistry , copolymer , polymer , adsorption , physics , optics
The present study describes the development and validation of a new method based on a matrix solid‐phase dispersion (MSPD) sample preparation procedure followed by GC‐MS for determination of acrylamide levels in coffee (ground coffee and brewed coffee) and coffee substitute samples. Samples were dispersed in C 18 sorbent and the mixture was further packed into a preconditioned custom‐made ISOLUTE bilayered SPE column (C 18 /Multimode; 1 g + 1 g). Acrylamide was subsequently eluted with water, and then derivatized with bromine and quantified by GC‐MS in SIM mode. The MSPD/GC‐MS method presented a LOD of 5 μg/kg and a LOQ of 10 μg/kg. Intra and interday precisions ranged from 2% to 4% and 4% to 10%, respectively. To evaluate the performance of the method, 11 samples of ground and brewed coffee and coffee substitutes were simultaneously analyzed by the developed method and also by a previously validated method based in a liquid‐extraction (LE) procedure, and the results were compared showing a high correlation between them.