Premium
Isolation and Molecular Characterization of Multidrug‐Resistant Strains of Escherichia coli and Salmonella from Retail Chicken Meat in Japan
Author(s) -
Ahmed Ashraf M.,
Shimabukuro Hirofumi,
Shimamoto Tadashi
Publication year - 2009
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2009.01291.x
Subject(s) - salmonella , isolation (microbiology) , escherichia coli , multiple drug resistance , microbiology and biotechnology , biology , food science , bacteria , drug resistance , gene , genetics
Sixty‐nine Escherichia coli and 10 Salmonella isolates, recovered from retail chicken meat in Hiroshima prefecture, Japan, were assayed for antimicrobial susceptibility, the presence of integrons and antimicrobial resistance genes. Twenty‐eight out of 69 (40.6%) of E. coli and all 10 Salmonella isolates were exhibited multidrug resistance phenotypes. The most commonly reported resistance phenotypes were against ampicillin, streptomycin, spectinomycin, kanamycin, tetracycline, and trimethoprim/sulfamethoxazole. PCR screening for integrons showed that 8 (11.6%) of the E. coli isolates were positive for the class 1 integrons and 1 isolate (1.4%) was positive for the class 2 integrons. Among the 10 Salmonella isolates, 9 were positive for class 1 integrons and none was positive for class 2 integrons. The identified antibiotic resistance gene cassettes within the class 1 integrons were dfrA1 , dfrA7 , aadA1 , aadB , and catB3 , while dfrA1 , sat2 , and aadA1 were identified within class 2 integron. The β‐lactamase resistance gene bla TEM‐1 was identified in 12 (17.3%) of E. coli isolates and in only one of the Salmonella isolates. The bla CMY‐2 gene, encoding AmpC β‐lactamase, was detected in 16 (23.2%) of the E. coli isolates only. Conjugation experiments demonstrated that there was plasmid‐mediated transfer of bla CMY‐2 and bla TEM‐1 . These results highlighted the role of retail chicken meat as a potential source for multidrug‐resistant strains of E. coli and Salmonella . To the best of our knowledge, this is the 1st report of isolation and molecular characterization of multidrug‐resistant strains of E. coli from retail chicken meat in Japan.