Premium
Antimicrobial Activity of Lactoperoxidase System Incorporated into Cross‐Linked Alginate Films
Author(s) -
Yener Fatih Y.G.,
Korel Figen,
Yemenicioğlu Ahmet
Publication year - 2009
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2009.01057.x
Subject(s) - pseudomonas fluorescens , antimicrobial , chemistry , escherichia coli , lactoperoxidase , bacteria , incubation , abts , food science , bacterial growth , microbiology and biotechnology , nuclear chemistry , biochemistry , enzyme , antioxidant , peroxidase , biology , organic chemistry , genetics , gene , dpph
In this study, the antimicrobial effect of lactoperoxidase (LPS) incorporated alginate films was investigated on Escherichia coli (NRRL B‐3008), Listeria innocua (NRRL B‐33314), and Pseudomonas fluorescens (NRRL B‐253) in presence of different concentrations of H 2 O 2 (0.2, 0.4, and 0.8 mM) and KSCN (1, 2, and 4 mM). The incorporation of 70 nmol ABTS/min/cm 2 LPS into alginate films gave 0.66 to 0.85 nmol ABTS/min/cm 2 enzyme activity at 0.2 to 0.8 mM H 2 O 2 concentration range. The antimicrobial activity of LPS system on target bacteria changed according to the concentrations of KSCN and H 2 O 2 . The growth of all tested bacteria was prevented for a 6‐h period by applying LPS system in presence of 0.4 or 0.8 mM H 2 O 2 and 4 mM KSCN. At 0.8 mM H 2 O 2 and 4 mM KSCN, the LPS system also inhibited growth of L. innocua and P. fluorescens for a 24‐h incubation period, whereas E. coli growth could not be inhibited for 24 h under these conditions. At 0.2 mM H 2 O 2 and 1 to 4 mM KSCN, a considerable inhibitory effect was obtained only on P. fluorescens . The decreasing order of the resistance of studied bacteria to LPS system is as follows: E. coli , L. innocua , and P. fluorescens . The developed antimicrobial system has a good potential for use in meat, poultry, and seafood since alginate coatings are already used in these products. Further studies are needed to test the LPS incorporated edible films in real food systems.