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Antioxidative and Anti‐Inflammatory Protection of Oleanolic Acid and Ursolic Acid in PC12 Cells
Author(s) -
Tsai ShihJei,
Yin M.C.
Publication year - 2008
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2008.00864.x
Subject(s) - chemistry , lactate dehydrogenase , oleanolic acid , ursolic acid , superoxide dismutase , glutathione , catalase , antioxidant , glutathione peroxidase , pharmacology , hydrogen peroxide , tumor necrosis factor alpha , biochemistry , endocrinology , enzyme , medicine , pathology , chromatography , alternative medicine
PC12 cells were used to examine the in vitro antioxidative and anti‐inflammatory effects of oleanolic acid (OA) and ursolic acid (UA). PC12 cells were pretreated with OA or UA at 20 and 40 μM and followed by exposure of hydrogen peroxide (H 2 O 2 ) or 1‐methyl‐4‐phenylpyridinium ion (MPP + ) to induce cell injury. Results showed that H 2 O 2 ‐ or MPP + ‐treatment significantly decreased cell viability and increased lactate dehydrogenase (LDH) release ( P < 0.05). The pretreatment from OA or UA significantly and concentration‐dependently reduced subsequent H 2 O 2 ‐ or MPP + ‐induced cell death and LDH release ( P < 0.05). Either H 2 O 2 ‐ or MPP + ‐treatment significantly increased malonyldialdehyde (MDA) formation, decreased glutathione (GSH) content, and diminished glutathione peroxidase (GPX), catalase, and superoxide dismutase (SOD) activities ( P < 0.05). The pretreatment from OA or UA significantly retained GSH, and reversed H 2 O 2 ‐ and MPP + ‐induced impairment in catalase and SOD activities ( P < 0.05), and decreased MDA formation ( P < 0.05). Either H 2 O 2 ‐ or MPP + ‐treatment significantly elevated interleukin‐6 (IL‐6) and tumor necrosis factor (TNF)‐α levels ( P < 0.05). The pretreatments from OA or UA significantly attenuated subsequent H 2 O 2 ‐ or MPP + ‐induced release of IL‐6 and TNF‐α ( P < 0.05). Based on the observed antioxidative and anti‐inflammatory activities from OA and UA, these 2 compounds were potent agents against neurodegenerative disorder.