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Study of the Biosynthesis of 1‐Octen‐3‐ol Using a Crude Homogenate of Agaricus bisporus in a Bioreactor
Author(s) -
Morawicki R.O.,
Beelman R.B.
Publication year - 2008
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2007.00660.x
Subject(s) - agaricus bisporus , mushroom , bioreactor , yield (engineering) , chemistry , linoleic acid , chromatography , food science , pulmonary surfactant , biochemistry , organic chemistry , fatty acid , materials science , metallurgy
1‐Octen‐3‐ol and 10‐oxo‐ trans ‐8‐decenoic acid are metabolites of the breakdown of linoleic acid (LA) by mushroom enzymes. These compounds can be produced in a bioreactor using a crude mushroom homogenate and the exogenous addition of LA and oxygen. The factors' duration of blending, mushroom–buffer ratio, effect of a surfactant, whole against partially clarified reaction broths, purity of LA, and utilization of stumps instead of whole mushrooms were studied for their effect on reaction yield using a 1‐L bioreactor. The results showed the feasibility of using the more inexpensive 60%‐pure LA instead of the 99%‐pure LA even when a yield loss was involved. Waste stumps could be used instead of whole mushrooms with a yield decline of 26%.