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Influence of Sodium Glycocholate on Production of Conjugated Linoleic Acid by Cells of Lactobacillus reuteri ATCC 55739
Author(s) -
RomanNunez M.,
CuestaAlonso E. P.,
Gilliland S. E.
Publication year - 2007
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2007.00347.x
Subject(s) - lactobacillus reuteri , chemistry , conjugated linoleic acid , linoleic acid , food science , sodium , bacteria , biochemistry , chromatography , lactobacillus , biology , fatty acid , fermentation , organic chemistry , genetics
The possible influence of a bile salt on production of conjugated linoleic acid (CLA) by Lactobacillus reuteri ATCC 55739 was evaluated. Cells of the lactobacilli grown in MRS broth with and without linoleic acid (LA, 0.2%) were harvested and washed. The washed cells were added to buffer containing 0.2% LA and incubated 18 h at 37 °C. The cells, which had been grown without LA, transformed LA into CLA (mainly c9t11‐C18:2) better than did those cells grown with it. When sodium glycocholate (0.3%) was added to the washed cell suspensions, about the same level of CLA was formed as in its absence regardless of whether or not the cells had been grown in broth supplemented with free LA. Thus, glycocholate that occurs in humans did not influence production of CLA by resting cells of the lactobacilli.