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Compositional Study on Rice Bran Oil after Lipase‐Catalyzed Glycerolysis and Solvent Fractionations
Author(s) -
Lee J. H.,
Yu F.,
Vu P. L.,
Choi M. S.,
Akoh C. C.,
Lee K. T.
Publication year - 2007
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2007.00299.x
Subject(s) - fractionation , chemistry , hexane , chromatography , rice bran oil , bran , solvent , fraction (chemistry) , acetone , lipase , yield (engineering) , organic chemistry , enzyme , raw material , materials science , metallurgy
Rice bran oil (RBO) was modified through lipase‐catalyzed glycerolysis. After 48 h reaction, the reactant (RBO‐G, solved in hexane) containing 0.14 mg/mL of MAG, 0.19 mg/mL of DAG, and 0.93 mg/mL of TAG was obtained. Extending the reaction to 72 h resulted in 0.37 mg/mL of DAG with concomitant reduction in TAG (0.68 mg/mL). Two solvent fractionation methods, independent and sequential fractionation, were performed with acetone and hexane at 0, −8, −14, or −35 °C. The fraction with most unsaturated fatty acids (ΣUFA) was liquid fraction from independent fractionation at −35 °C (−35In) from hexane, showing 88.3%ΣUFA content. Nevertheless, when yield (wt%) was considered, the highest amount of UFA was obtained from 0In (liquid fraction from independent fractionation at 0 °C) with hexane, resulting in 82.3%ΣUFA with 97.9 wt% recovery. Normal‐phase HPLC was conducted for the compositional study of RBO‐G. Overall, solid fractions from sequential fractionation at 0 °C (0SeSo) and independent fractionation at −35 °C (−35InSo) with hexane contained the high concentration of total MAG and DAG, ranging from 0.94 to 1.35 (mg/mL).