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Effects of NaCl Concentration on Salting‐in and Dilution During Salting‐out on Soy Protein Fractionation
Author(s) -
Deak N. A.,
Murphy P. A.,
Johnson L. A.
Publication year - 2006
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2006.00028.x
Subject(s) - dilution , salting , chemistry , fraction (chemistry) , salting out , fractionation , chromatography , yield (engineering) , extraction (chemistry) , soy protein , food science , materials science , aqueous solution , organic chemistry , thermodynamics , physics , metallurgy
Glycinin and β‐conglycinin are the main storage proteins in soybeans that can be fractionated by using alkali extraction, SO 2 , salting‐in with NaCl, salting‐out by dilution and pH adjustment to produce a glycinin‐rich fraction, a β‐conglycinin–rich fraction, and an intermediate fraction, which is a mixture of the two proteins. Two different strategies were employed to optimize the procedure to achieve high efficiency in recovering the β‐conglycinin–rich fraction. The first strategy was to optimize salting‐in effects of NaCl, and the effects of NaCl concentration on the yields and purities of the protein fractions were investigated. The maximum protein yield of the β‐conglycinin–rich fraction was obtained at 500 mM NaCl, but at the expense of purity. The optimum NaCl concentration was 250 mM, at which good protein yield (18.5%) and purity (84.5%) were achieved. At higher NaCl concentrations, the protein yields of the intermediate fractions were significantly lower, and the protein loss in the whey fraction increased. The second strategy was to improve the salting‐out step for the β‐conglycinin–rich fraction. At 0‐ and 0.5‐fold dilution, the purities and yields of the β‐conglycinin–rich fractions were significantly lower than at 1.0‐ and 2.0‐fold dilution. There were no differences in protein yields or purities when using 1.0‐ or 2.0‐fold dilution. According to these results, the recommended NaCl concentration for the salting‐in step is 250 mM and the dilution factor for salting‐out is 1.0.