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Rapid Detection of Fish Major Allergen Parvalbumin by Surface Plasmon Resonance Biosensor
Author(s) -
Lu Y.,
Ohshima T.,
Ushio H.
Publication year - 2004
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1750-3841.2004.tb18013.x
Subject(s) - sardine , allergen , surface plasmon resonance , parvalbumin , chemistry , chromatography , monoclonal antibody , serial dilution , detection limit , antibody , allergy , biology , fish <actinopterygii> , immunology , fishery , materials science , medicine , nanotechnology , alternative medicine , pathology , neuroscience , nanoparticle
Seafood allergy is a common and major cause of food allergy in adults. In recent years, seafood allergy has become a serious problem with the increase of seafood consumption. To develop a rapid allergen detection method based on the affinity of antigen‐antibody interaction, fish major allergen, parvalbumin, was used for kinetic analysis by a surface plasmon resonance (SPR) biosensor. Anti‐parvalbumin murine monoclonal antibody (MAb) EG8 was immobilized onto a carboxymethyl dextran (CMD) surface. By the injection of various concentrations of purified carp parvalbumin (CPa), a standard curve and the affinity constants (K D and k * ) for the MAb EG8‐CPa model system were determined. In addition, kinetic data were also obtained by the injection of serial dilutions of extracts from seafood products: sardine fish cake ( tsumire ) and dried skipjack tuna (katsuonut). Sardine tsumire and katsuonut contained 0.11 mg/kg and 0.39 mg/kg parvalbumins, respectively, where affinity constants K D and k * were almost similar among paralbumins from different sources. In the SPR system, the allergen can be detected only for 5 min according to the allergen‐MAb binding interaction. Consequently, by the use of a SPR biosensor, kinetic analysis based on the allergen specific MAb would be a rapid and powerful tool for allergen detection and quantification.