Open AccessCD45 Isoform Expression in Microglia and Inflammatory Cells in HIV‐1 Encephalitis
Author(s) -
CosenzaNashat Melissa A.,
Kim MeeOhk,
Zhao MengLiang,
Suh HyeonSook,
Lee Sunhee C.
Publication year - 2006
Publication title -
brain pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.986
H-Index - 132
eISSN - 1750-3639
pISSN - 1015-6305
DOI - 10.1111/j.1750-3639.2006.00027.x
Subject(s) - microglia , gene isoform , in vivo , antibody , cd8 , microbiology and biotechnology , biology , chemistry , immunology , inflammation , immune system , gene , biochemistry
CD45 is a membrane tyrosine phosphatase that modulates the function of the hematopoietic cells. In vitro , agonist antibodies to CD45RO or CD45RB isoforms have been shown to suppress microglial activation, but whether microglia in vivo express these isoforms in HIV encephalitis (HIVE) is unknown. Brain sections from control and HIVE were immunostained for CD45 isoforms using exon‐specific antibodies (RA, RB, RC and RO). RA and RC were limited to rare lymphocytes, while RB expression was robust in microglia and inflammatory cells. RO was low in control microglia, but increased in HIVE. RO was also localized to macrophages and CD8+ T cells. Targeting CD45 in vivo with isoform‐specific antibodies remains a therapeutic option for neuroinflammatory diseases.