Open AccessCharacterization of Neural Cell Lines Derived from SV40 Large T‐Induced Primitive Neuroectodermal Tumors
Author(s) -
Weggen Sascha,
Bayer Thomas A.,
Koch Anke,
Salewski Holger,
Scheidtmann KarlHeinz,
Pietsch Torsten,
Wiestler Otmar D.
Publication year - 1997
Publication title -
brain pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.986
H-Index - 132
eISSN - 1750-3639
pISSN - 1015-6305
DOI - 10.1111/j.1750-3639.1997.tb01059.x
Subject(s) - synaptophysin , glial fibrillary acidic protein , nestin , neural cell adhesion molecule , biology , neurofilament , cell adhesion molecule , neural cell , cell culture , pathology , microbiology and biotechnology , progenitor cell , cell adhesion , cell , immunohistochemistry , neural stem cell , stem cell , immunology , medicine , genetics
We recently reported intriguing properties of neural tumors generated by retrovirus‐mediated transfer of the SV40 large T antigen into fetal rat brain transplants. Histopathologically, these neoplasms displayed characteristic features of primitive neuroectodermal tumors (PNET) and exhibited a striking potential for migration into the host brain. In the present study, four cell lines were derived from these PNETs and characterized. Two lines with an immature phenotype expressed the embryonal form of the neural cell adhesion molecule and nestin. They showed spheroid formation and delicate cell processes. The remaining cell lines had a flat, epitheloid appearance and were immunoreactive for synaptophysin, neurofilament proteins and glial fibrillary acidic protein. These cells constitute valuable tools to study the cellular origin(s) and molecular basis of PNETs, differentiation of neural progenitors and tumor cell migration in the brain.