Open AccessMyelinated Axons Demonstrated in the CNS and PNS by Anti‐Neurofilament Immunoreactivity and Luxol Fast Blue Counterstaining
Author(s) -
Yao DaLin,
Komoly Samuel,
Zhang QianLin,
Webster Henry deF.
Publication year - 1994
Publication title -
brain pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.986
H-Index - 132
eISSN - 1750-3639
pISSN - 1015-6305
DOI - 10.1111/j.1750-3639.1994.tb00815.x
Subject(s) - luxol fast blue stain , neurofilament , pathology , central nervous system , peripheral nervous system , anatomy , biology , immunohistochemistry , medicine , myelin , neuroscience
A method which demonstrates myelinated axons in the central and peripheral nervous systems will be described. Paraffin, frozen or semi‐thin epoxy‐embedded sections were immunostained first with a monoclonal antibody raised against a 200 kilo‐Dalton neurofilament protein and then counterstained with Luxol fast blue