Open AccessDemonstration of Amyloid Deposits and Neurofibrillary Changes in Whole Brain Sections
Author(s) -
Braak Heiko,
Braak Eva
Publication year - 1991
Publication title -
brain pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.986
H-Index - 132
eISSN - 1750-3639
pISSN - 1015-6305
DOI - 10.1111/j.1750-3639.1991.tb00661.x
Subject(s) - polyethylene glycol , amyloid (mycology) , silver stain , nucleation , staining , pathology , extracellular , chemistry , biophysics , materials science , biology , biochemistry , medicine , organic chemistry
Selective and sensitive silver staining of extracellular amyloid deposits and intraneuronal neurofibrillary changes can be applied to 50–150 μ.m thick polyethylene glycol sections and/or 5–15 μ.m thick paraffin sections. The silver techniques take advantage of physical development of the nucleation sites thus permitting tight control of the entire procedure. Both techniques can be applied to routinely fixed autopsy material. They do not require particular skills and considerably facilitate processing of large numbers of sections through entire hemispheres of the human brain.