Open AccessProtective efficacy of crude virus‐like particle vaccine against HPAI H5N1 in chickens and its application on DIVA strategy
Author(s) -
Park JaeKeun,
Lee DongHun,
Youn HaNa,
Kim MyeongSeob,
Lee YuNa,
Yuk SeongSu,
Lim TaeHyun,
Jang JunHyuk,
Kwon JungHoon,
Kim ByoungYoon,
Kang SangMoo,
Seong BaikLin,
Lee JoongBok,
Park SeungYong,
Choi InSoo,
Song ChangSeon
Publication year - 2013
Publication title -
influenza and other respiratory viruses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.743
H-Index - 57
eISSN - 1750-2659
pISSN - 1750-2640
DOI - 10.1111/j.1750-2659.2012.00396.x
Subject(s) - influenza a virus subtype h5n1 , diva , virology , biology , immunogenicity , vaccination , culling , highly pathogenic , virus , immunization , virus like particle , antibody , immunology , recombinant dna , herd , zoology , biochemistry , gene
Background Currently, Asian lineage highly pathogenic avian influenza (HPAI) H5N1 has become widespread across continents. These viruses are persistently circulating among poultry populations in endemic regions, causing huge economic losses, and raising concerns about an H5N1 pandemic. To control HPAI H5N1, effective vaccines for poultry are urgently needed. Objective In this study, we developed HPAI virus‐like particle (VLP) vaccine as a candidate poultry vaccine and evaluated its protective efficacy and possible application for differentiating infected from vaccinated animals (DIVA). Methods Specific pathogen‐free chickens received a single injection of HPAI H5N1 VLP vaccine generated using baculovirus expression vector system. Immunogenicity of VLP vaccines was determined using hemagglutination inhibition (HI), neuraminidase inhibition (NI), and ELISA test. Challenge study was performed to evaluate efficacy of VLP vaccines. Results and Conclusions A single immunization with HPAI H5N1 VLP vaccine induced high levels of HI and NI antibodies and protected chickens from a lethal challenge of wild‐type HPAI H5N1 virus. Viral excretion from the vaccinated and challenged group was strongly reduced compared with a mock‐vaccinated control group. Furthermore, we were able to differentiate VLP‐vaccinated chickens from vaccinated and then infected chickens with a commercial ELISA test kit, which offers a promising strategy for the application of DIVA concept.