Open AccessEffect of Seawater on the Activity of Antibiotics Against Vibrios Isolated from the Hemolymph of Cultured Pacific White Shrimp
Author(s) -
Costa Renata Albuquerque,
Colares Lorena Pontes,
Lima Rayza Araújo,
Fernandes Vieira Regine Helena Silva dos,
de Sousa Oscarina Viana
Publication year - 2012
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2012.00590.x
Subject(s) - microbiology and biotechnology , biology , shrimp , vibrio , litopenaeus , tetracycline , antibiotics , ampicillin , furazolidone , oxytetracycline , penicillin , imipenem , seawater , food science , bacteria , antibiotic resistance , fishery , ecology , genetics
The purpose of this study was to evaluate the effect of seawater (SW) on the activity of antibiotics belonging to 10 families (aminoglycosides, aminopenicillins, carbapenems, β‐lactams, chloramphenicols, monobactams, nitrofurans, quinolones, sulfonamides, and tetracyclines) against Vibrio strains isolated from hemolymph of Pacific white shrimp, Litopenaeus vannamei , farmed in Northeastern Brazil and standard strain Vibrio cholera ATCC 19582. Susceptibility of the strains to antibiotics was determined by disk diffusion method and the minimum inhibitory concentration was determined by macrodilution method. The media Mueller–Hinton agar and broth used in the above methods were diluted in distilled water (control, 1% NaCl, pH 7.5) and SW (2.5% NaCl, pH 7.5). The antibiotics most affected by dilution in SW were tetracycline, penicillin, cephalothin, aztreonam, ampicillin, and imipenem, as indicated by a considerable increase in the number of strains classified as intermediate or resistance. Thus, in this study, the efficiency of these antibiotics on Vibrio strains was found to be reduced by contact with SW.