Open AccessEffect of Three Probiotics Administered Through Live Feed on Digestive Enzyme Activity in California Halibut, Paralichthys californicus , Larvae
Author(s) -
ZacariasSoto Magali,
Lazo Juan Pablo,
Viana Maria Teresa
Publication year - 2011
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2011.00470.x
Subject(s) - biology , pepsin , trypsin , chymotrypsin , paralichthys , lipase , digestive enzyme , larva , digestion (alchemy) , probiotic , enzyme assay , food science , enzyme , biochemistry , ecology , fishery , bacteria , chemistry , genetics , chromatography , fish <actinopterygii>
The objective of this study was to evaluate the effect of probiotics as digestive system maturation promoters in larvae of the California halibut, Paralichthys californicus, during ontogeny. Three commercial probiotics: Levucell ® , Bactocell ® , and live yeast, were supplied at a concentration of 50 mg/L, using enriched rotifers and Artemia nauplii as vectors. Total and specific activities of trypsin, leucine‐aminopeptidase (LAP), lipase, chymotrypsin, and pepsin were assessed throughout the development. Growth in terms of length was not significantly different between treatments. Total pepsin and chymotrypsin activity were significantly higher at the end of the experiment, 46‐d posthatch (dph) in larvae supplied with Bactocell. However, no significant effect of the probiotic was detected for trypsin, lipase, and LAP. On the basis of the results, California halibut larvae seemed to have adequate alkaline proteolytic activity early in development, whereas acid proteolytic activity was not detected until the complete formation of a functional stomach. The feeding protocol and probiotics evaluated were unable to improve the morphological development of digestive system or the digestive enzyme activity early on. Nevertheless, the increase in activity of pepsin and chymotrypsin observed at 46 dph suggests a potential use of these probiotics once the metamorphosis is completed, and thus warrants further evaluation.