Open AccessCharacterization of Ornithine Decarboxylase, a Potential Selective Breeding Marker, from Small Abalone, Haliotis Diversicolor
Author(s) -
Li Weidong,
You Weiwei,
Chen Weiyu,
Qin Ji,
Huang Zhaobin,
Ke Caihuan,
Wang Yiquan
Publication year - 2010
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2010.00414.x
Subject(s) - abalone , biology , china , fishery , microbiology and biotechnology , law , political science
The enzyme ornithine decarboxylase (ODC) is the first limiting enzyme in polyamine biosynthesis and plays an important role in biological processes. Although recognized as a potential biomarker in vertebrates, it has not been well studied in aquatic animals. Identification of ODC is a prerequisite for its application as a selective breeding marker in small abalone, Haliotis diversicolor . In this article, we focused on the in vitro characterization of the ODC gene from small abalone. The expressed product showed decarboxylase activity for l ‐ornithine and was inhibited by DL‐α‐difluoromethylornithine hydrochloride hydrate. ODC was expressed in a wide range of tissues at varying levels, with the lowest in the hepatopancreas and the highest in the antennae. ODC is potentially useful as a molecular marker for small abalone breeding and can also facilitate further investigations on its biological functions in mollusks.